Journal: PLOS ONE

Article Title: New oncogenic functions of LINE1 retroelement as a ceRNA for tumor suppressive microRNA miR-126 on ENPP5

doi: 10.1371/journal.pone.0286814

Figure Lengend Snippet: (A) Hsa-miR-126 levels in BRCA public database. (B) Hsa-miR-126-5p expression in HBC cell lines compared to normal MCF-10A cells. (C) Kaplan-Meier plot showed that highly expressed hsa-miR-126 associated with better overall survival in HBC patients. (D) cfa-miR-126 expression was down-regulated in CMT cell lines when compared with normal mammary gland. (E) ENPP5 expression was significantly up regulated in BRCA public database. (F) ENPP5 expression was also significantly up-regulated in HBC related cell lines of SKBR-3, MCF-7 and MDA-MB-231. Log2-transformed expression was used (G) miR-126-5p (mimic) transfection significantly reduced the ENPP5 mRNA levels. (H) miR-126-5p transfection (mimic) also significantly reduced the luciferase activity conjugated with the 3’UTR of ENPP5. (I) The luciferase activity conjugated with the 3’UTR of ENPP5 was significantly increased by the overexpression of LINE in MDA-MB-231 cells. (J) ENPP5 mRNA was significantly increased by LINE overexpression in MCF-10A cells. *, **, *** and **** indicate significance p<0.05, 0.01, 0.001, and 0.0001, respectively.

Article Snippet: ENPP5 3’UTR sequence was amplified with MCF-10A genomic DNA, digested with AsiSi and XhoI enzyme (NEB, MA, USA), and inserted into pEZX-GA02 vector (GeneCopoeia, MD, USA).

Techniques: Expressing, Transformation Assay, Transfection, Luciferase, Activity Assay, Over Expression

Journal: Journal of inorganic biochemistry

Article Title: Cellular Uptake of Protic Ruthenium Complexes is Influenced by pH Dependent Passive Diffusion and Energy Dependent Efflux

doi: 10.1016/j.jinorgbio.2019.110922

Figure Lengend Snippet: The Change in pH e over 48 h in Various Cell Lines and Uptake of Complex 3 .

Article Snippet: HeLa, MCF7, MDA-MB-231, and MCF10A cell lines were purchased from ATCC.

Techniques:

Journal: Journal of inorganic biochemistry

Article Title: Cellular Uptake of Protic Ruthenium Complexes is Influenced by pH Dependent Passive Diffusion and Energy Dependent Efflux

doi: 10.1016/j.jinorgbio.2019.110922

Figure Lengend Snippet: MCF10A, MCF7, MDA-MB-231, and HeLa cells were administered with 50 μM of 3A and were studied by flow cytometry. (a – d) Flow cytometry contour plots of cell size and granularity were detected using forward scatter (FSC) and side scatter (SSC), respectively. Cell debris was detected at bottom left corner for all cell lines (≤ 2.0K SSC, ≤ 2.0K FSC). The population on the left indicates cells with smaller sizes, i.e., shrunk cells (FSC 2.0K-4.0K), whereas the population on the right indicates cells with normal sizes (FSC ≥5.0K). (e – h) Flow cytometry histogram analysis shows fluorescence intensity which is proportional to the Ru concentration. Shrunk and normal cells correspond to the same populations from a-d.

Article Snippet: HeLa, MCF7, MDA-MB-231, and MCF10A cell lines were purchased from ATCC.

Techniques: Flow Cytometry, Fluorescence, Concentration Assay

Journal: Journal of inorganic biochemistry

Article Title: Cellular Uptake of Protic Ruthenium Complexes is Influenced by pH Dependent Passive Diffusion and Energy Dependent Efflux

doi: 10.1016/j.jinorgbio.2019.110922

Figure Lengend Snippet: Cellular uptake of 3A as a function of concentration (x axis) is proportional to the mean fluorescence intensity (MFI) ± SD (y axis). Cells were treated with 0 – 100 μM of 3A in the dark. (a) MCF10A. (b) MCF7. (c) MDA-MB-231. (d) HeLa. The no drug control (-) shows the inherent fluorescence for the cells in the absence of 3A.

Article Snippet: HeLa, MCF7, MDA-MB-231, and MCF10A cell lines were purchased from ATCC.

Techniques: Concentration Assay, Fluorescence, Control

Journal: Journal of inorganic biochemistry

Article Title: Cellular Uptake of Protic Ruthenium Complexes is Influenced by pH Dependent Passive Diffusion and Energy Dependent Efflux

doi: 10.1016/j.jinorgbio.2019.110922

Figure Lengend Snippet: Representative flow cytometry analysis of the influence of light on cellular uptake of 50 μM of 3A. Fluorescence intensity is proportional to the Ru concentration in cells. (a) MCF10A. (b) MCF7. (c) MDA-MB-231. (d) HeLa. This data suggests that light does not cause increased uptake.

Article Snippet: HeLa, MCF7, MDA-MB-231, and MCF10A cell lines were purchased from ATCC.

Techniques: Flow Cytometry, Fluorescence, Concentration Assay

Journal: Journal of inorganic biochemistry

Article Title: Cellular Uptake of Protic Ruthenium Complexes is Influenced by pH Dependent Passive Diffusion and Energy Dependent Efflux

doi: 10.1016/j.jinorgbio.2019.110922

Figure Lengend Snippet: Representative flow cytometry analysis of cellular uptake of 50 μM of 3A as measured by MFI in the dark under normal metabolism (control in blue) and under metabolic inhibition conditions (with deoxyglucose and oligomycin in red). (a) MCF10A. (b) MCF7. (c) MDA-MB-231. (d) HeLa. Fluorescence intensity is proportional to the Ru concentration in cells.

Article Snippet: HeLa, MCF7, MDA-MB-231, and MCF10A cell lines were purchased from ATCC.

Techniques: Flow Cytometry, Control, Inhibition, Fluorescence, Concentration Assay